Osaze Odemwingie is set for a two-week spell on the sidelines after sustaining a hamstring injury in training.
The news will come as a blow to the Nigerian international who endured a frustrating 2014/15 campaign after suffering knee ligament damage in the 1-0 triumph over Manchester City last August.
He did however make a full recovery back from that to feature in the latter stages of last season, and built upon that during the Potters’ hectic pre-season schedule.
Odemwingie stepped off the bench against Liverpool in last Sunday’s 2015/16 curtain-raiser, but will take no part in Saturday’s trip to White Hart Lane.
“Unfortunately Peter has got a hamstring strain and is likely to miss the next couple of weeks,” Hughes said at the pre-match press conference.
“It’s disappointing for him because he has worked tremendously hard throughout pre-season.”
Meanwhile, former Barcelona star Bojan has been pencilled in to take part in the under 21s fixture against Wolves at the Britannia Stadium on Saturday morning, as he continues his rehabilitation back from a long-term injury.
“He won’t travel with the first team on Saturday, but he is incredibly close to being fit and available,” Hughes added.
“He is going to play for the under 21s on Saturday to build up his fitness levels. We feel he is better served getting game time rather than sitting on the bench.”